With the development of the epidemic, the problem of “false negatives” in nucleic acid testing has become increasingly prominent. According to a report from Southern Weekend, a patient in Hangzhou has been tested negative for 6 nucleic acid tests, and it was not tested positive until the 7th time. Pointed out that the current positive rate of nucleic acid tests is about 30%-40%. A negative test cannot rule out new coronavirus pneumonia, and multiple negative tests cannot be ruled out.
It is imminent to solve the problem of “false negatives” in nucleic acid testing. The reasons for “false negative” results are very complicated, such as whether the sample collection is accurate, the degree of infection of patients, the sensitivity of detection reagents, etc. This article mainly introduces the selection of virus storage tubes for accurate detection. degree of influence.
Novel coronavirus nucleic acid can be detected in nasopharyngeal swabs, sputum, lower respiratory tract secretions, blood, feces and other specimens. Because of the high requirements for obtaining lower respiratory tract samples such as bronchoalveolar lavage fluid (bronchoscopy, etc.) and the high risk of infection, it is generally used in clinical practice for patients with severe illness. In addition, the clinical manifestations of this new coronavirus infection are often dry cough, deep phlegm Liquid sampling also faces difficulties. Therefore, for this new coronavirus nucleic acid detection and sampling, nasal and throat swabs are the most commonly used methods.
