|2×EasyTaq PCR SuperMix (-dye)/(+dye)||1 ml||5×1 ml||15×1 ml||6×80 ml|
|Nuclease-free Water||1 ml||5 ml||3×5 ml||
EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. For 2×EasyTaq® PCR SuperMix (-dye), the PCR products which contain dA overlangs at the 3' end can be directly cloned to pEASY®-T vectors. For 2×EasyTaq® PCR SuperMix (+dye), the PCR products can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning. Please note, PCR products using this SuperMix are not suitable for polyacrylamide gel electrophoriesis (PAGE).