Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.
• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.
at -20°C for two years
Dry ice (-70℃)
|Component||FM201-01 (10 rxns)|
|2×TransStart® FastPfu Fly PCR SuperMix||1 ml|
|DMT Enzyme (10 units/μl)||30 μl|
|2×Assembly Mix||50 μl|
|DMT Chemically Competent Cell||10×50 μl|
|Nuclease-free Water||1 ml|