|EasyTaq® DNA Polymerase for PAGE||2500 U||2500 U×4|
|10×EasyTaq® Buffer for PAGE||1.2 ml ×5||1.2 ml×20|
|2.5 mM dNTPs||- / 800 μl×5||- / 800 μl×20|
|6×DNA Loading Buffer||1 ml||
1 ml ×4
EasyTaq® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.
Short fragment PCR
at -20 ℃ for two years